・IFCC Transferable Method
・High Linearity ( Range 3 ~ 3000 U/L) & High Repeatability
・Little interference by coexisting substances

This reagent is intended for the in vitro quantitative determination of γ-Glutamyltransferase (γ-GT) in human serum or plasma.

γ-Glutamyltransferase measurements are used in the diagnosis and treatment of liver diseases such as alcoholic cirrhosis and primary and secondary liver tumors.
Elevated serum γ -glutamyltransferase (γ-GT) is found in all forms of liver disease.
It is more sensitive than alkaline phosphatase, the transaminases, and leucine aminopeptidase (LAP) in detecting obstructive jaundice, cholangitis, and cholecystitis. γ-GT levels rise earlier in liver disease and to higher values than LAP or 5’-nucleotidase levels.
Moderate elevations are seen in infectious hepatitis. However, elevated γ-GT levels have also been noted in chronic alcoholism, diabetes, and certain neurological disorders.
Normal levels of γ-GT are seen in skeletal diseases; thus γ-GT in serum can be used to ascertain whether a disease is skeletal or hepatobiliary.

Values	Causes
High	Alcohol consumption, Alcohol abuse, Alcoholic liver disease, Liver neoplasms, Viral hepatitis, Fatty liver disease
Low	Familial intrahepatic cholestasis type 1

This method is based on the recommendations of the International Federation of Clinical Chemistry (IFCC).
γ-GT catalyzes the transfer of the γ-glutamyl group from the substrate, L-γ-glutamyl-3-carboxy-4-nitroanilide (Glupa-C), to glycylglycine (Gly-Gly), yielding 5-amino-2-nitrobenzoate.
The change in absorbance at 405/570 nm is due to the formation of 5-amino-2-nitrobenzoate and is directly proportional to the γ-GT activity in the sample.

Individual instrument applications are available on request.

Reagent 1: Glupa-C pH 6.5 (25°C)
Reagent 2: Glycylglycine pH 7.9 (25°C)

Reagent is liquid, ready to use.

Storage 　: 2-10°C
Shelf Life : 16 months (Prior to use)
Stability of the opened reagents:
Reagent 1: stable for at least 1 month (2-10°C).
Reagent 2: stable for at least 1 month (2-10°C).

The following performance data were obtained on a Hitachi 7180 clinical analyzer.

Method Comparison
Comparison studies were carried out using another similar commercially available method. The following results were obtained.



Sensitivity
The sensitivity was evaluated by reading the change in absorbance for purified water sample and serum samples with known concentrations.
The results indicated that γGT-L showed little or no reagent drift on a zero sample.
Under the reaction condition described, 200 U/L γGT activity gives a ΔABS/min of 0.017~0.035.

Linearity


Precision (within-run)
(U/L)

n=20	Serum I	Serum II
Mean	27.9	139.4
Min	27	138
Max	28	141
R	1	3
SD	0.357	0.663
CV (%)	1.28	0.48

Interfering Substances